Solving renewable energy source selection problems using a q-rung orthopair fuzzy-based integrated decision-making approach

This paper proposes an integrated decision-making framework for the systematic selection of a renewable energy source (RES) from a set of RESs based on sustainability attributes. A real case study of RES selection in Karnataka, India, using the framework is demonstrated, and the results are compared with state-of-the-art methods. The main reason for developing this framework is to handle uncertainty and vagueness effectively by reducing human intervention. Systematic selection of RESs also reduces inaccuracies and promotes rational decision-making. In this paper, q-rung orthopair fuzzy information is adopted to minimize subjective randomness by providing a flexible and generalized preference style. Further, the study found systematic approaches for imputing missing values, calculating attributes’ and decision-makers’ weights, aggregation or preferences, and prioritizing RESs, which are integrated into the framework. Comparing the proposed framework with state-of-the-art-methods shows that (i) biomass and solar are suitable RESs for the process under consideration in Karnataka, (ii) the proposed framework is consistent with state-of-the-art methods, (iii) the proposed framework is sufficiently stable even after weights of attributes and decision makers are altered, and (iv) the proposed framework produces broad and sensible rank values for efficient backup management. These results validate the significance of the proposed framework

Impact of glucocorticoid receptor density on ligand-independent dimerization, cooperative ligand-binding and basal priming of transactivation: a cell culture model

Glucocorticoid receptor (GR) levels vary between tissues and individuals and are altered by physiological and pharmacological effectors. However, the effects and implications of differences in GR concentration have not been fully elucidated. Using three statistically different GR concentrations in transiently transfected COS-1 cells, we demonstrate, using co-immunoprecipitation (CoIP) and fluorescent resonance energy transfer (FRET), that high levels of wild type GR (wtGR), but not of dimerization deficient GR (GRdim), display ligand-independent dimerization. Whole-cell saturation ligand-binding experiments furthermore establish that positive cooperative ligand-binding, with a concomitant increased ligand-binding affinity, is facilitated by ligand-independent dimerization at high concentrations of wtGR, but not GRdim. The down-stream consequences of ligand-independent dimerization at high concentrations of wtGR, but not GRdim, are shown to include basal priming of the system as witnessed by ligand-independent transactivation of both a GRE-containing promoter-reporter and the endogenous glucocorticoid (GC)-responsive gene, GILZ, as well as ligand-independent loading of GR onto the GILZ promoter. Pursuant to the basal priming of the system, addition of ligand results in a significantly greater modulation of transactivation potency than would be expected solely from the increase in ligand-binding affinity. Thus ligand-independent dimerization of the GR at high concentrations primes the system, through ligand-independent DNA loading and transactivation, which together with positive cooperative ligand-binding increases the potency of GR agonists and shifts the bio-character of partial GR agonists. Clearly GR-levels are a major factor in determining the sensitivity to GCs and a critical factor regulating transcriptional programs